A practical reference on GHRH analog: what it is, how it behaves, what the literature reports, and where the honest uncertainties sit.
Reviewed 2025-10-25. Anything still debated is marked as such rather than presented as settled.
The two variants differ dramatically in how long they persist in circulation. The form lacking the albumin-binding group has a plasma half-life measured in tens of minutes, comparable to the natural hormone fragment. The version carrying the drug affinity complex binds albumin and shows a half-life of roughly six to eight days in human studies. That figure comes from small trials that tracked hormone levels over extended periods. The physiological consequences of sustained versus pulsatile stimulation are still debated and the literature does not settle the point.
Lyophilized peptide powder is comparatively stable when kept dry, cold, and protected from light. Once dissolved, the molecule is vulnerable to deamidation, oxidation, and aggregation, with the rate depending on pH, buffer composition, and temperature. Alkaline conditions and repeated freeze-thaw cycles accelerate loss of the intact peptide. The methionine present in the native sequence is a known oxidation site, which is one reason it was replaced in the modified fragment. Suppliers typically recommend cold storage of solutions and use within a short window.
CJC-1295 is the name used for a synthetic peptide modeled on growth hormone-releasing hormone, the hypothalamic signal that prompts the pituitary to release growth hormone. The compound was described by a Canadian drug discovery company in the mid-2000s as a long-acting research tool. Two closely related molecules share the name in practice: one carries a drug affinity complex, or DAC, group, and one does not. The distinction matters because the two behave differently in circulation.
The peptide backbone corresponds to GRF(1-29), the first 29 residues of native growth hormone-releasing hormone, which retains most of the receptor-activating activity of the full-length molecule. Four substitutions distinguish the analog from the natural sequence: D-alanine at position 2, glutamine at position 8, alanine at position 15, and leucine at position 27. These changes slow cleavage by dipeptidyl peptidase IV, the enzyme that degrades native hormone in plasma within minutes. The outcome is improved enzymatic stability combined with a still brief residence time when no additional modification is present.
| Property | Value | Notes |
|---|---|---|
| Molecular mass | Approximately 3.4 to 3.6 kDa | Depends on whether the affinity complex is attached |
| Appearance | White to off-white lyophilized powder | Freeze-dried solid, often in a sealed vial |
| Solubility | Soluble in water and aqueous buffers | Dissolution rate varies with pH and buffer salt |
| Typical storage | Below minus 20 degrees Celsius, dry and dark | Dissolved material is usually kept cold and used promptly |
| Common analytical methods | Reversed-phase HPLC and mass spectrometry | Peptide mapping and amino acid analysis add sequence detail |
The compound emerged from work at a Canadian biotechnology firm in the early 2000s. Early human studies examined its effect on growth hormone and insulin-like growth factor 1 in healthy volunteers and in people with HIV-associated fat redistribution. Reports described sustained increases in both markers after a single injection. Development did not advance to regulatory approval, and the clinical programme was later discontinued. The molecule is now encountered mainly as a research chemical rather than a marketed medicine.
Two forms circulate in research settings and are frequently confused. One carries the drug affinity complex and is often written as CJC-1295 with DAC; the other lacks that group and is usually called modified GRF(1-29). The two share the same core sequence but differ sharply in how long they persist in blood. Products labelled only as CJC-1295 normally refer to the version carrying the complex. Documentation that omits the distinction leaves the intended molecule ambiguous.
Identity and purity are established with reversed phase high performance liquid chromatography coupled to mass spectrometry. The chromatographic step separates the target peptide from truncated sequences and deletion products, while the mass measurement confirms the expected molecular weight to within a fraction of a dalton. Because the two common variants differ by the presence of the linker, mass alone can distinguish them in the unconjugated state. Amino acid analysis and peptide mapping are used when sequence level confirmation is required.
Lyophilized material is generally stable for extended periods when held at minus twenty degrees Celsius or below and protected from moisture and light. In solution the peptide is more labile; bond hydrolysis, aggregation and oxidation of susceptible residues all proceed faster at ambient temperature. Repeated freeze and thaw cycles should be avoided because they promote clumping and loss of soluble material. The conjugated variant adds a further consideration, since the maleimide group can hydrolyze in aqueous buffer and lose its ability to react with albumin.
Laboratory handling centers on minimizing exposure to water, heat and oxygen before use. Working solutions are typically prepared in sterile water or a mild buffer, and any residual particulate matter is removed by filtration. When the powder dissolves slowly, a small proportion of acetonitrile or dilute acetic acid is sometimes added as a co-solvent. Containers are kept sealed and desiccated between uses. Records of lot number, reconstitution date and storage conditions support later comparison of results across experiments.
"Naram-Sin, the mighty, king of Agade, when the four quarters together revolted against him, through the love which the goddess Astar showed him, he was victorious in nine battles in one in 1 year, and the kings whom they (the rebels[?]) had raised (against him), he captured. In view of the fact that he protected the foundations of his city from danger, (the citizens of his city requested from Astar in Eanna, Enlil in Nippur, Dagan in Tuttul, Ninhursag in Kes, Ea in Eridu, Sin in Ur, Samas in Sippar, (and) Nergal in Kutha, that (Naram-Sin) be (made) the god of their city, and they built within Agade a temple (dedicated) to him. As for the one who removes this inscription, may the gods Samas, Astar, Nergal, the bailiff of the king, namely all those gods (mentioned above) tear out his foundations and destroy his progeny."
=== After the Islamabad Memorandum === Following the resumption of hostilities on July 8, ending the Islamabad Memorandum, initial punitive strikes against Iran had gradually morphed into an attempt to "break Iran’s grip on the strait".
=== 5. Alveolar bone === In advanced stages of attachment loss, inflammation-mediated bone resorption reduces alveolar crest height. This bone loss may present as horizontal or angular defects, compromising periodontal ligament support and further accelerating clinical attachment loss.
Sources: en.wikipedia.org
Technological change is the largest cause of long-term economic growth. Throughout human history, energy production was the main constraint on economic development, and new technologies allowed humans to significantly increase the amount of available energy. First came fire, which made a wider variety of foods edible, and made it less physically demanding to digest them. Fire also enabled smelting and the use of tin, copper, and iron tools, used for hunting or tradesmanship. Then came the agricultural revolution: humans no longer needed to hunt or gather to survive, and began to settle in towns and cities, forming more complex societies, with militaries and more organized forms of religion. Technologies have contributed to human welfare through increased prosperity, improved comfort and quality of life, and medical progress, but they can also disrupt existing social hierarchies, cause pollution, and harm individuals or groups. Recent years have brought about a rise in social media's cultural prominence, with potential repercussions on democracy, as well as economic and social life. Early on, the Internet was seen as a "liberation technology" that would democratize knowledge, improve access to education, and promote democracy. Modern research has turned to investigate the Internet's downsides, including disinformation, polarization, hate speech, and propaganda. Since the 1970s, technology's impact on the environment has been criticized, leading to a surge in investment in solar, wind, and other forms of clean energy.
The main objective of food refrigeration and/or freezing is to preserve the quality and safety of food materials. Refrigeration and freezing contribute to the preservation of perishable foods, and to the conservation of some food quality factors, such as visual appearance, texture, taste, flavor and nutritional content. Freezing food slows the growth of bacteria that could potentially harm consumers.
A set of nuclides with equal proton number (atomic number), i.e., of the same chemical element but different neutron numbers, are called isotopes of the element. Particular nuclides are still often loosely called "isotopes", but the term "nuclide" is now considered the correct one in the general case when no specific element (Z value) encompasses them. In similar manner, a set of nuclides with equal mass number A, but different atomic number, are called isobars (isobar = equal in weight), and isotones are nuclides of equal neutron number but different proton numbers. Likewise, nuclides with the same neutron excess (N − Z) are called isodiaphers. The name isotone was derived from the name isotope to emphasize that in the first group of nuclides it is the number of neutrons (n) that is constant, whereas in the second the number of protons (p). See Isotope#Notation for an explanation of the notation used for different nuclide or isotope types. Nuclear isomers are members of a set of nuclides with equal proton number and equal mass number (thus making them by definition the same isotope), but different states of excitation. An example is the two states of the single isotope 9943Tc shown among the decay schemes. Each of these two states (technetium-99m and technetium-99) qualifies as a different nuclide, illustrating one way that nuclides may differ from isotopes (an isotope may consist of several different nuclides of different excitation states).
In response to the 2008–09 Israel–Gaza conflict Galloway instigated the Viva Palestina aid convoy to the Gaza Strip in January 2009. "There is a kind of intifada among the youth. They are determined to act", he told Cole Moreton writing for The Independent, adding, "We say, 'Don't be lured by the siren voices of separatism and extremism – join with us and express your anger politically, in a way that will be peaceful, non-violent, and not cost you your life, but will not cost other people their lives either". By mid-February, the organisation said it had raised over £1,000,000 for humanitarian aid in four weeks, although the Charity Commission later found the true figure to be £180,000. On 14 February 2009, Galloway and hundreds of volunteers launched the convoy comprising approximately 120 vehicles intended for use in the Strip, including a fire engine donated by the Fire Brigades Union (FBU), 12 ambulances, a boat and trucks full of medicines, tools, clothes, blankets and gifts for children. The 5,000-mile route passed through Belgium, France, Spain, Morocco, Algeria, Tunisia, Libya and Egypt. The convoy arrived in Gaza on 9 March, accompanied by approximately 180 extra trucks of aid donated by Libya's Gaddafi Foundation. On 10 March 2009, Galloway announced at a press conference in Gaza City attended by several senior Hamas officials: "We are giving you now 100 vehicles and all of their contents, and we make no apology for what I am about to say.
Sources: en.wikipedia.org
The version carrying the affinity complex bears a maleimide group that binds serum albumin, which extends its circulation time to several days. The version without it lacks this group and clears within roughly half an hour. The two are chemically related but behave very differently once in the body.
In common usage the name without the affinity complex is often equated with MOD GRF 1-29, a fragment carrying four stabilizing substitutions. Strictly speaking, the term originally referred to the albumin-binding version. The overlap in naming causes frequent ambiguity in both informal and technical writing.
Reversed-phase chromatography separates the peptide from related impurities and yields a purity estimate. Mass spectrometry confirms the molecular mass and detects modifications such as oxidation. Sequence-level checks rely on peptide mapping or amino acid analysis when stronger confirmation is needed.
No. It is a laboratory-made analog of growth hormone-releasing hormone. The natural hormone is a 44-residue peptide, while the analog is built on a shorter 29-residue fragment.